No, the Roku player cannon seamlessly loop content. You can cause video to play on repeat, but it will start a new playback and rebuffer at the start each time. There are a number of other reasons why Roku isn't the right choice for commercial/industrial applications like yours. I'd suggest checking out BrightSign (
www.brightsign.biz) for what you want to do. It's related to Roku, including using BrightScript for development, but the feature set and hardware design is tailored to commercial applications.
Patrick